sequences downstream of the gag - pol junction . open reading frame in the gag gene and Rous sarcoma virus RNA stability requires
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Expression of the Rous sarcoma virus pol gene by ribosomal frameshifting.
The pol gene of Rous sarcoma virus is positioned downstream of the gag gene in a different, briefly overlapping reading frame; nevertheless, the primary translation product of pol is a gag-pol fusion protein. Two mechanisms, ribosomal frameshifting and RNA splicing, have been considered to explain this phenomenon. The frameshifting model is supported by synthesis of both gag protein and gag-pol...
متن کاملThermodynamic stability and statistical significance of potential stem-loop structures situated at the frameshift sites of retroviruses.
RNA stem-loop structures situated just 3' to the frameshift sites of the retroviral gag-pol or gag-pro and pro-pol regions may make important contributions to frame-shifting in retroviruses. In this study, the thermodynamic stability and statistical significance of such secondary structural features relative to others in the sequence have been assessed using a newly developed method that combin...
متن کاملRous Sarcoma Virus RNA Stability Element Inhibits Deadenylation of mRNAs with Long 3′UTRs
All retroviruses use their full-length primary transcript as the major mRNA for Group-specific antigen (Gag) capsid proteins. This results in a long 3' untranslated region (UTR) downstream of the termination codon. In the case of Rous sarcoma virus (RSV), there is a 7 kb 3'UTR downstream of the gag terminator, containing the pol, env, and src genes. mRNAs containing long 3'UTRs, like those with...
متن کاملComplementation studies with Rous sarcoma virus gag and gag-pol polyprotein mutants.
Avian retroviruses (with the notable exception of spleen necrosis virus) express their protease (PR) both in their gag and their gag-pol polyprotein precursors, in contrast to other retroviruses, notably, the mammalian retroviruses, in which PR is encoded in the gag-pol polyprotein or in a separate reading frame as a gag-pro product. The consequence is that the avian PR is expressed in stoichio...
متن کاملProbing the mechanism of ribosomal frameshifting on viral RNAs.
Introduction Over the last few years it has been recognized that a number of eukaryotic viruses use a highly efficient, programmed ribosomal frameshift mechanism to control expression of their replicases (reviewed in [ 11). Ribosomal frameshifting is a directed change in translational reading frame that allows the production of a single protein from two (or more) overlapping genes, and was firs...
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تاریخ انتشار 1993